High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast
نویسندگان
چکیده
منابع مشابه
High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast.
Total internal reflection fluorescence microscopy is widely used to confine the excitation of a complex fluorescent sample very close to the material on which it is supported. By working with high refractive index solid supports, it is possible to confine even further the evanescent field, and by varying the angle of incidence, to obtain quantitative information on the distance of the fluoresce...
متن کاملObservation of Nanoscale Refractive Index Contrast via Photoinduced Force Microscopy
Near-field optical microscopy (NSOM) is a scanning probe technique that allows optical imaging of sample surfaces with nanoscale resolution. Generally, all NSOM schemes rely on illuminating the sample surface and collecting the localized scattered light resulting from the interaction of the microscopes nanoscale probe with the sample surface in the illuminated region. Currently, a new set of na...
متن کاملPlasmonic Refractive Index Sensor with High Figure
A plasmonic refractive index (RI) sensor based on metal-insulator-metal (MIM) 11 waveguide coupled with concentric double rings resonator (CDRR) is proposed and investigated 12 numerically. Utilizing the novel supermodes of the CDRR, the FWHM of the resonant wavelength 13 can be modulated, and a sensitivity of 1060 nm/RIU with high figure of merit (FOM) 203.8 is realized 14 in the near-infrared...
متن کاملHigh Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on ...
متن کاملA New Structure of Mmi Polymer Thermo- Optic Switch with a High Refractive Index Contrast
The 2 × 2 MMI polymer thermo-optic switch in a high refractive index contrast (0.102) with a new structure design is realized. This device was fabricated using standard fabrication techniques such as coating, photolithography, and dry etching. A crosstalk level of −36.2 dB has been achieved. Meanwhile the extinction ratio of 36.1 dB has been achieved in this device. The polarization dependent l...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2001
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.241208698